Remedial Instruction

Remedial Instruction in English 1. Define and enumerate the procedure of the following: a. augmentative picture( Communication) device †¢These devices of technology can range from: †¢A board with pictures representing a student’s daily needs †¢Words, phrases, sentences symbolically represented on a communication display as photographs, line drawings, tangible objects or letters †¢Electronic speech synthesizers (prerecorded speech, or segments of words, computers and portable electronic devices. †¢Augmentative communication devices help individuals in producing and/or understanding speech. b. Bean bag tossing -Cornhole, also known as bean bag toss, corn toss, baggo or bags, is a lawn game in which players take turns throwing corn hole bags at a raised platform with a hole in the far end. A bag in the hole scores 3 points, while one on the platform scores 1 point. Play continues until a team or player reaches the score of 21. Procedure †¢Bean bag to ssing matches are broken down into innings or frames [1] of play. During each inning, every player throws four bags. A player may deliver the bag from either the left or right pitcher's box, but, in any one inning, all bags must be delivered from the same pitcher's box.It is possible that both players can throw from the same pitcher's box. Also, the player gets a three foot box to throw in. Each player must deliver the bag within twenty seconds. The time starts when the player steps onto the pitcher's box with the intention of pitching. The player who scored in the preceding inning pitches first in the next inning. If neither pitcher scores, the contestant or team who pitched last in the preceding inning pitches first in the next inning. Note: one foot cannot pass the back of the board while tossing the corn bag, otherwise the point does not count. Bean bag tossing can be played as either doubles or singles. In doubles play, four players split into two teams. One member from each te am pitches from one cornhole platform and the other members pitch from the other. The first side of players alternate pitching bags until both players have thrown all four of their bags, then the players pitching from the opposing cornhole board continue to alternate in the same manner until all four of their bags are delivered and the inning or frame is completed. In singles play, two players play against each other.Delivery is handled in the same manner as doubles play. Both contestants pitch from the same cornhole platform and alternate their pitches until all of their bags have been pitched, completing the inning or frame. c. Sensory putty †¢Therapy Putty is the clinician's all-purpose answer for children who are nervous, anxious, distracted, or withdrawn in therapy. Most children can't resist Therapy Putty—It can be squeezed, stretched, twisted, and pulled into an endless assortment of shapes. It gives children an engaging yet undemanding activity that helps them ca lm down and sit still.And it often serves as an effective ice-breaker, leading the child into relaxed and productive discussion. †¢Therapy Putty is formulated to ensure long-lasting plasticity. It contains only organic, NON-BLEED colors, and it is odorless and nontoxic. It is also ideal for developing hand-muscle strength and increasing endurance. To gently increase range of motion or reduce stiffness, use Extra-Soft Tan. As strength increases, gradually increase the resistance of putty. Varying resistances are indicated by color Procedure 1.Stretch the Theraputty out and hide coins or small objects in it. Then roll it up into a ball. Have the child pull the putty apart and find all the objects you put in there. 2. Roll the putty out into a long thin snake. Have the child use their index finger to flatten the snake all along its body. Then, using their pincer grip (index finger to thumb) have them squeeze the putty back up. I should now look like a spiky dinosaur! 3. Holding th e putty in one hand, have the child pinch pieces of the putty off at a time with each finger opposing the thumb d. Sandtray technique Sandtray therapy is considered an expressive and dynamic form of psychotherapy that facilitates expression of thoughts through metaphor and symbols, according to the Sandtray Therapy Institute. The technique aims at healing individuals by allowing them to get in touch with their inner processes and past experiences. The Institute touts its ability to let people become who they are meant to be, rather than being limited by what they were taught when they were younger. †¢is a form of expressive therapy that is also known as sandplay and the world technique.It was developed by Margaret Lowenfeld, Goesta Harding, De Domenico, Charlotte Buehler, Bolgar, Fisher, Ruth Bowyer, and Dora Kalff. This non-verbal method of therapy is often used with children, but can be applied to adults, teens, couples, families, and groups as well. Sand tray therapy allows a client to construct their own microcosm using miniature toys and colored sand. The newly created microcosm then acts as a reflection of the client’s own life and allows them the opportunity to resolve conflicts, remove obstacles, and to connect to their inner being and recognize the beauty of their own soul as they begin to accept themselves.Procedure – One of the most common techniques used in sand tray therapy is the world technique. This approach involves the use of miniatures. Clients are encouraged to use miniature toys, figurines and objects in the sand in way they choose. They can add water to the sand, and place the miniatures in the sand tray in any order. The design of the sand tray is guided by their imagination and their subconscious. The result is a microcosm of their inner world. The world within the sand tray is expressed through symbolism and metaphor, and may not even make immediate sense to the client.But aided by the therapist, a client, even a chi ld, can begin to recognize the relationship between the creation in the sand and their own inner world. -The humanistic approach is another common strategy applied in sand tray therapy. Clinicians who use this technique rely solely on the client to find solutions to their problems, using the sand as a tool for healing. Through creative expression, a client is able to manifest in sand things they would otherwise not be able to vocalize or address in traditional therapy.The therapist treats the client as whole and healed, knowing that the process of sand tray therapy allows the client to find the answers that are already within them. e. Pad cast †¢a digital recording of music, news or other media that can be downloaded from the internet to a portable media player. The term originated from â€Å"P. O. D. †, meaning Portable On Demand, and â€Å"cast†, relating to the term broadcast †¢Anyone can create a podcast. All over the world, people are creating podcasts o n subjects ranging from movies, to technology, to music, to politics and whatever else you can think of.This is new original content made by passionate people who want to share their creativity with the world. †¢The cost to start podcasting is so low that anyone can do it. Most podcasters are everyday people like you and me. They could be talking to you driving in their car, sitting in their living room or speaking at a conference. You get to glimpse into their life and into their interests. †¢Podcasters are creating very raw and real content and listeners are responding. Free from corporate radio and broadcast regulations, you can create whatever kind of show you can imagine. Some podcasts are â€Å"talk show† style. Others introduce you to the latest bands and music. With podcasts you can stay current on the news, get a glimpse into someone’s life, listen to move reviews and the list goes on. †¢Most podcasts are made by people who are very passionate a bout their subject. Passion is infectious and interesting to listen to. Since the birth of podcasting, a huge variety of shows have shown up on the internet. †¢In addition, many major media outlets are also now offering podcasts. You can listen to NPR, Rush Limbaugh and many major news outlets by podcast. f. Math Manipulatives In math classrooms today, teachers are using manipulatives to help students learn mathematics. Manipulative materials are any concrete objects that allow students to explore an idea in an active, hands-on approach. Manipulatives can be almost anything –blocks, shapes, spinners or even paper that is cut or folded. †¢The power of using manipulatives is that they let the student connect mathematical ideas and symbols to physical objects, thus promoting better understanding. For example, students in grade 5 must learn about decimal numbers. Students ma ke a common mistake nwhen comparing 0. 7 and 0. 6, thinking that 7 tenths is a smaller number th an 56 hundredths. This is because students think that a two-digit number, such as 56hundredths is larger than a one-digit number, 7 tenths. That â€Å"rule† works for wholenumbers, but not decimals. If the students are asked to build these numbers using amanipulative such as base ten blocks, they can immediately see that 7 tenths is larger than56 hundredths. They connect the model to the concept of the size of the numbers. With many experiences building and representing using manipulatives, students can deepen their understanding of abstract math concepts.It can also be tools to help students solve problems. By using physical models to represent their thinking, they can move and adapt the materials as they explore possiblesolutions to problems G. Raised line paper †¢One way to assist students with difficulty in writing is by offering raised-line paper. The lines on this paper are embossed, which raises them slightly. This provides both visual and tactile cues for student s who have difficulty with letter formation, spacing and â€Å"staying in the lines. † As students write, they will â€Å"feel† the lines both with their fingers and through the motion of their pencils.This may help students to form letters and words more accurately, increasing their feeling of success with writing tasks. †¢When using this kind of tool in your classroom, it’s best to provide it for everyone if possible. This will help class projects or bulletin boards to look uniform in appearance. More importantly, kids with disabilities will have an opportunity to see that they are using the same materials as their peers. Already acutely aware that they struggle with writing, they don’t need to be self-conscious about using different paper than everyone else.In order to save money, kids can use scrap paper or regular paper for writing or dictating rough drafts, and then use the raised-line paper for final copies if necessary Procedure †¢Just p repare the tools(the particular paper) and let the students follow the lines I the paper. h. Sparkle †¢A spelling word game than has been played a lot in classrooms is Sparkle. The basic game goes like this. The person in charge of calling out the words can be the teacher or a student. When the first word is called out, the first person starts spelling it, saying only the first letter.The second person says the second letter and so on down the line. Whoever says the last letter of the word turns to the next person and says â€Å"sparkle† or any other word of your choosing, like â€Å"Whoo-hoo† or â€Å"Gotcha. † The person who would have been next is out, because he or she got â€Å"sparkled†. †¢The game continues with the leader calling out another word. Any time a student misspells a word, that student is out. Some teachers have a student sit down if they are not paying attention and do not know it is their turn or do not remember the previous letter. This helps improve listening skills.Procedure 1. The class forms a circle. 2. The teacher says a list word. 3. The first student says the first letter of the word. 4. The next in line says the second letter. 5. The student next in line says the third letter. This continues until all letters of the list word have been spoken. 6. The student in line AFTER the last letter, then says the list word. 7. The next student in line says â€Å"SPARKLE† and is OUT, and returns to his seat. 8. Anytime a student says the wrong letter, he is out and returns to his seat. 9. I usually give the winner a cap eraser, pencil grip, or something like that. . Adaptive reading materials Multisensory techniques are frequently used for students withLearning Disabilities (LD) Multisensory teaching techniques and strategies stimulate learning by engaging students on multiple levels. They encourage students to use some or all of their senses to: †¢Gather information about a task; †¢Link information to ideas they already know and understand; †¢Perceive the logic involved in solving problems; †¢Learn problem-solving steps; †¢Tap into nonverbal reasoning skills; †¢Understand relationships between concepts; and †¢Learn information and store it for later recall.

Hypersensitivity Case Study Essay

*Poison Ivy- The body’s immune system is normally in the business ­ of protecting us from bacteria, viruses, and other foreign invaders that can make us sick. But when urushiol from the poison ivy plant touches the skin, it instigates an immune response, called dermatitis, to what would otherwise be a harmless substance. Hay fever is another example of this type of response; in the case of hay fever, the immune system overreacts to pollen, or another plant-produced substance. Here’s how the poison ivy response occurs. Urushiol makes its way down through the skin, where it is metabolized, or broken down. Immune cells called T lymphocytes (or T-cells) recognize the urushiol derivatives as a foreign substance, or antigen. They send out inflammatory signals called cytokines, which bring in white blood cells. Under orders from the cytokines, these white blood cells turn into macrophages. The macrophages eat foreign substances, but in doing so they also damage normal tissue, resulting in the skin inflammation that occurs with poison ivy.  ­ The allergic reaction to poison ivy is known as delayed hypersensitivity. Unlike immediate hypersensitivity, which causes an allergic reaction within minutes of exposure to an antigen, delayed hypersensitivity reactions don’t emerge for several hours or even days after the exposure. Most people don’t have a reaction the first time they touch poison ivy, but develop an allergic reaction after repeated exposure. Everyone has a different sensitivity, and therefore a slightly different reaction, to poison ivy. Sensitivity usually decreases with age and with repeat exposures to the plant. *Transfusing a person of Type A Blood with Type B Blood- ABO blood type system The ABO system consists of A, B, AB, and O blood types. People with type A have antibodies in the blood against type B. People with type B have antibodies in the blood against type A. People with AB have no anti-A or anti-B antibodies. People with type O have both anti-A and anti-B antibodies. People with type AB blood are called universal recipients, because they can receive any of the ABO types. People with type O blood are called universal donors, because their blood can be given to people with any of the ABO types. Mismatches with the ABO and Rh blood types are responsible  for the most serious, sometimes life-threatening, transfusion reactions. So unless the person is RH- there usually isn’t that big of a reaction when An Type A is given Type B blood. These reactions may be mild or severe. Most mild reactions are not life-threatening when treated quickly. Even mild reactions, though, can be frightening. Severe transfusion reactions can be life-threatening, but this is very rare. Mild allergic reactions may involve itching, hives, wheezing, and fever. Severe reactions may cause anaphylactic shock. Exposure to a field of Ragweed- Ragweed allergy, similar to other pollen allergies, may include symptoms of allergic rhinitis, allergic conjunctivitis and allergic asthma. Symptoms caused by ragweed pollen allergy would be expected to start during August and September and last until October or November, depending on the climate. So stay out of a field of Ragweed during those times a year if you experience allergy or hypersensitivity around ragweed. *Unsuccessful Kidney Transplant- Transplant rejection is when transplanted tissue is rejected by the recipient’s immune system, which destroys the transplanted tissue. Transplant rejection can be lessened by determining the molecular similitude between donor and recipient and by use of immunosuppressant drugs after transplant. Hyper-acute rejection occurs a few minutes after the transplant when the antigens are completely unmatched. The tissue must be removed right away so the recipient does not die. This type of rejection is seen when a recipient is given the wrong type of blood. For example, a person given type A blood when he or she is type B.

Rethink What You Drink Essay

Between an 8oz glass of orange juice to a 24oz can of soda, one person can drink between six to eighteen teaspoons of sugar. One may not think that a can of soda a day will hurt a person greatly; but that person is wrong. If you consume one non-diet soda, sugary sport or energy drink, even juice, it can increase your risk of heart disease, obesity, diabetes and high blood pressure, even leading to chronic problems down the road. Even drinking a Five-Hour Energy or Monster in the morning everyday can lead to a caffeine related death. Keeping up homework, going to practice after school and maintaining a social life, a day being a teenager can be draining. We stay up late texting or studying for a test at the last minute, and sleep is not our number one priority. Yes, we wake up the next morning wishing we did not stay up so late, but there is always a cure for that. Almost one third of 12 to 24 year olds said they regularly down an energy drink in the morning. There is nothing wrong with consuming a Starbucks coffee or Five-Hour energy shot in the morning if it is the only thing that will keep us going. The sugar and caffeine in juice, energy drinks and coffee is one of the main factors that keep us teens moving at the pace we do. There are juices, diet sodas and energy drinks that have an okay amount of sugar and caffeine in them to where we could have one a day, and never be harmed. The National Health and Nutrition Exanimation Survey and Nutrition Services worker, Cheri Collier states, â€Å"A â€Å"sugary drink† is known as fruit drinks, soda, energy and sport drinks and sweetened bottles of water and also coffee beverages† (Gann) The consumption of sugary drinks remains high today. In fact, over a half of the American population has a sugary drink almost every day. Lisa Cimperman, registered dietitian for University Hospitals Case Medical Center said, â€Å"Many people consume too many calories each day; Liquid calories don’t make you feel full. Causing people to eat and drink more. † (Theiss) When someone has so much sugar in their system, it can lead to obesity and diabetes. It is shown that 25. 8 million Americans have diabetes (8% of population) and 191 million (2/3) are overweight/obese. (Gann) Not to say that a soda a day can lead to this, but when you are consuming mass amounts of sugar, you will become a part of that 25. 8 million. Men and women are both at risk for developing heart disease. Men who drink one sugar-sweetened beverage daily have a 20% higher risk of chronic heart disease then men who drink none. A study put on by the Harvard School of Public Health surveyed 43,000 men throughout 22 years, and results form 18,000 men were demographically similar. Results in American Heart Association journal stated: â€Å"Drinking 12oz of regular soda, fruit drinks and other sugar sweetened beverages daily was associated with higher risk of heart disease. † Dr. Frank Hu, Professor of nutrition and epidemiology at Harvard School of Public Health said that the findings were notable. The same study was put on for a group of 89,000 women, and it was shown that women who drank one or less than two sugary drinks per day had a 23% increased risk of heart attack and it was four times as likely to have a chronic heart problem down the road. (Jaslow) Studying for tests, going out to parties, finishing research papers late into the night, maintaining a social life and working, life of a college student can be draining. Balancing everything usually takes a toll on one of our most critical bodily functions, sleep. So how do these college students wake up in the morning and go to class with energy? A study done by the University of Ohio stated that 74% of undergraduate students turn to energy supplements in the form of drinks or shots. A single Monster contains 14 teaspoons of sugar and a Five-Hour Energy contains over 8,000 percent of the daily-recommended intake of vitamin B12 and over 2,000 percent of vitamin B6. They say that the rush of energy one feels when consuming the drinks is due to the amount of sucralose, an artificial sweetener over 600 times sweeter than sugar and you will also see over 500 milligrams of caffeine in some drinks. So what happens to people who drink these energy drinks and Five-Hour Energy shots? According to Medicine Plus in 2007, almost 6,000 caffeine related deaths occurred in people under the age of 19, and since then, the numbers have continued to rise(Silva). When you come to thinking about drinking a nice cold 8oz soda, remember that you are putting a toxic drug into your body. You could one day have had too many sodas and be sitting at home with heart disease or diabetes. Remember not to over load yourself with a caffeine overloaded energy drink or shot. Try resorting to diet drinks with not as much sugar, or 100% fruit drinks. â€Å"Our soda habit is something we have total control over. There are a lot of things that keep us healthy that are hard work and difficult, but cutting back on sweetened drinks isn’t one of them† Dr. Stacey Rosen said, the chairman of cardiology at Long Island Jewish Medical Center in New Hyde Park, NY. Next time, rethink what you drink. Works Cited Jaslow, Ryan. â€Å"Sugary Drink Each Day Ups Men’s Heart Disease Risk by 20 Percent. â€Å"Www. cbsnews. com. CBS News, 14 Mar. 2012. Web. 3 Dec. 2012. . Gann, Carrie, and Larry Borges. â€Å"Study: One Sugary Drink Per Day Raises Risk of Heart Disease for Men. † Www. abcnews. com. ABC News, 12 Mar. 2012. Web. 3 Dec. 2012. . Reidt, Robert. â€Å"Sugary Drinks Can Be Hard on Heart: Study. â€Å"Www. healthyliving. msn. com. Healthy Day, 2 Mar. 2012. Web. 3 Dec. 2012. . Theiss, Evelyn. â€Å"Consumption of Sugar Drinks in the U. S. Remains High, Study Shows. â€Å"Www. cleveland. com. N. p. , 31 Aug. 2011. Web. 3 Dec. 2012. . Silva, Evan D. â€Å"Monsters v. Students; the Battle for Energy. † Lariat. Multimedia Editor, 22 Oct. 2012. Web. 15 Feb. 2013. .

Analyisis of Yell Group PLC Essay Example | Topics and Well Written Essays - 2500 words - 1

Analyisis of Yell Group PLC - Essay Example range of media like printed directories, local and national newspapers, and online directories and also provides business and residential information on phone. The main areas of operation of the group are United Kingdom and United States of America. (Company Overview, Worldscope) In the year 2006, the company posted a total sales figure of â‚ ¤1621.30 million. The operating income was â‚ ¤449.30 million, and the net income was â‚ ¤212.30 million. The total assets of the company in 2006 amounted to â‚ ¤3592.60 million as against the total liabilities of â‚ ¤2486.80million. The EBITDA is â‚ ¤493.90million. The key fundamentals of the company the company are given as under: The sales of the company increased from 670.51 million pounds in 2002 to 1285.30 million pounds in 2005. From 2002 to 2003 there was a 66.14% increase in sales. This increase in sales stabilized to 6.54% in 2004 and then increased to 8.29% in 2005. From 2002 to 2005 the cost of goods sold increased from 226.10 million pounds to 470.70 million pounds. The first year saw an increase of about 72.05%. This increase also stabilized to 11.39% in 2004 and 8.63% in 2005. The cost of goods sold from 2002 to 2500 decreased consistently, while the rate of increase in sales dipped to 6.54% from a high of 66.14% in 2003. The gross income and the operating income have also increased consistently from 2003 onwards. The increases were the highest from 2002 to 2003, and all the increases were over 50% in all the above measures. At the same time, the company has been trying to reduce its general, admin, selling expenses and other expenses. These expenses were again very high from 2002 to 2003, but ha ve steadily decreased since then. The earnings before interest and taxes increased by 59.46% in 2003 and decreased at the rate of 49.30% in 2004. It again showed a phenomenal increase of 161.15% in 2005. This trend of decrease and then increase was a result of an increase in the pretax extraordinary charge at the data 64.12%

C130 Aircraft Essay Example | Topics and Well Written Essays - 2000 words

C130 Aircraft - Essay Example The C-130 Aircraft also known as the Lockheed C-130 Hercules is a four-engine aircraft build by Lockheed during the 1950s. It is been serving since many years as a transport aircraft for military. This aircraft was originally developed as an assault transport. This was later used for a variety of missions such as: The C-130 aircraft is capable of traveling through rough, dirt strips. It is the basic mean of transport where paratroop and dropping of equipment is concerned for hostile regions all around the globe. It is the only aircraft that is in a process of continuous production for more than 50 years and have been participating in a huge number of missions of aid for humanity, military and civilian. With its original primary customer it has became the fifth most continuously used aircraft in the history, the first being the English Electric Canberra. (Posner, 2000, p. 1) The transports that were earlier used at the time of Korean World War II in June 1950 were C-119 Flying Boxcars, C-47 Sky trains and C-46 Commandos. However these were proved to be inadequate and insufficient to perform to the fullest and for a variety of purposes. After this realization, on February 02, 1951, the air force of the United States of America put forward a General Operating Requirement for a new transport. This requirement was introduced to a number of companies including: 1. Lockheed. 2. Martin. 3. Chase Aircraft. 4. Fairchild. 5. Douglas. 6. Northrop. 7. North American. 8. Boeing. 9. Airlifts Inc. (Stokes, 2000, p. 2) This new transport was required to have a capacity 92 passengers, also of 72 combat troops or 64 paratroopers. The range was required to be of 1300 miles and 2000 kilometers. Also it should be able to work well while having one of its engines turned off. Out of these companies only placed the tender of an accumulated number of ten designs for the required specifications including: 1. Boeing proposed one, 2. Lockheed proposed two, 3. Douglas proposed three, 4. Airlifts proposed one, and 5. Chase Aircraft proposed to design three under the specification The Lockheed design was developed and headed by Willis Hawkins. This was a proposal of 130 pages. There was close competition between the design proposals of Lockheed and that of Douglas. (Stokes, 2000, p. 3) The company won the contract on July 02, 1951 and was signed by the Company's Vice President and Chief Engineer Hall Hibbard. The first flight of the newly built YC-130 was carried out on August 23, 1954 from Burbank, California. This aircraft was piloted by Stanley Beltz and Roy Wimmer. Then later on the production of C-130 begins. It was started to being produced in Marietta Georgia. More than 2300 of C-130 were built at that time. Deliveries of the said started in December 1956. (Neely, 2000, p. 4) SPECIFICATIONS AND COST OF C-130 AIRCRAFT: The aircraft power plant includes Four Allison T56-A-15 turboprops, 4300 horsepower, each engine. The weight of the empty

Strategic Market, The Southwest Airline Model Research Paper - 1

Strategic Market, The Southwest Airline Model - Research Paper Example The employees at the company are trained to help customers and serve them genuinely with care in order to make customers happy; thus the company philosophy is translated into stockholder and customer happiness. The business model of the corporation is to eradicate the unnecessary frills provided by competitors; hence reducing the cost to clients. The industry reduces the cost by focusing on a lesser number of routes and only flying one type of plane; hence it has created a niche market in the aviation industry. Therefore, by choosing only to employ one type of aircraft, it was a very strategic choice that could enable the company to sustain their business in the global competitive business world. The Southwest airline industry have made significant efforts of employing effective strategic management policies and business model that can enable them to meet the demanding needs of customers in the targeted market. Michael Porter has attempted to reveal unique and valuable strategic position of an industry based on a tailored set of activities that can enable the company to achieve competitive advantage (Hill and Jones, 2009). Many industries have made considerable efforts of employing Porter’s five forces of competitive advantage in order to sustain their business in the global competitive market. The company manager of Southwest airline industry wanted to create a low cost aviation solution that can offer quality service to customers; hence enabling the company to sustain a competitive advantage. Therefore, the use of non-conventional models for low-cost and controlled solid growth for the airline has been among the major aspects for achieving competitiveness in the air line industry (Gross and Schroder, 2007). The industry also segments targeted market in varied ways including travelling short distances, frequent schedules, low cost fares, as well as, cost and value conscious customers. The other consists of

Research Report Essay Example | Topics and Well Written Essays - 1000 words - 3

Research Report - Essay Example g in mind that less that 65 percent of Australian tourist are locals hence the market needs to be invested in and promotions carried to promote international customers. A market share is a contributing factor towards any business achieving certain revenue. The report was design to determine the reason as to why Village Road show Theme Parks have been registering a decline in revenue from international customers. There exist models in which the theme parks should design and adapt tin order to keep their revenue flow at bay. The reports will analyse on the nature at which these models would prove effective. The base of the changing trend will be the year 2003. The negative effects of reduced international customers would be used as a means of innovating marketing strategies to increase the local customers and use the increased number to compensate the amount of revenue lost as a result of reduced number of international clients. There exist emerging trends in the Theme Parks in that the demand for services offered has led to competitors appreciating the market and investing. Village Road show Theme Parks are designed to offer a range of diversified services. The theme benefits from the economies of scale due to its diverse nature. The theme parks operate on the basis that both the local and international tourist would enjoy services provide in the parks. Revenue collected range differently in every theme park under the Village Road show Theme Parks brand name. The park boast of various parks and according to Australasian (2014), the launch of wet and wild Sydney Park, saw the theme park register a 6 million profit within week one of its inauguration. The revenue increased as compared to 2009. The year indicated higher number of local tourist as compared to international tourist, The management decision to venture into the Asian market was a positive move. According to March (2003), Australia was the preferred destination for many of the Asian tourist. Over the

The Western Treatment of Illness versus Alternative Therapies of Essay

The Western Treatment of Illness versus Alternative Therapies of Probiotics, Herbs and Cleansing Regimens - Essay Example Bacteria which are immune to the traditional treatments of antibiotics have become a serious issue for practitioners and the general public. Over-reliance on antibiotics by family doctors, and non-compliance by patients (usually through lack of understanding about the nature of antibiotics and bacteria) has produced infections such as Clostridium difficile, known as C. dif, which require very intensive therapies and are very resistant to treatment. It therefore makes sense for scientists to consider whether alternative therapies are of any benefit in providing an alternative to antibiotics. Complementary therapy such as herbs may actually cheaper than conventional medications. A study comparing standard anti-depressants with St John's Wort found that the side effects of the herb were only 10 percent that of the medications, and the herb was only one-fourth the cost of the anti-depressant. (Jonas, W. 209). Treatment of Helicobacter Pylori through antibiotics is : The side effects of antibiotics often result in a significant failure to complete the course - this has repercussions both in the duration of the illness, and the likelihood of the bacteria becoming resistant to treatment. Studies have shown that probiotics act rapidly (Mattila-Sandholm et all, 2000). In addition, it is widely acknowledged that patients are complacent about antibiotics, (Guardian 2005), and complementary therapy could provide a solution to that problem (Jonas, 208) Alternative therapies also provide a 'holistic' treatment, though some clinicians feel that it is nonsense to claim that conventional medicine does not treat the whole person (Baum, 1988)However, there is evidence that probiotics strengthen the immune system (Kailasapathy, Chin, 82; Senok, Ismaeel, and Botta, 959-960), providing long-term benefits for the patient. In cases where the patient is vulnerable to infection, this may be of particular interest. Problems with CAM Although many people take CAM without problems, there are safety issues which mean that alternative therapy is not benign. Firstly, the herbs themselves may be toxic in large doses (Silverstein et al, 162), they may be contra-indicated with conventional medication (Silverstein, 162; Jonas 209), and generally less well regulated (Senok, 959). There is also little hard evidence that CAM's are more effective than conventional treatments (Mattila-Sandholm, 394; Langmead, Rampton, 348), and despite some clinicians' feelings that CAM's have a beneficial effect, some studies seem to show the opposite (Gionchetti, et al, 492). The biggest concern over the use of CAM in treating bacterial diseases, however, is the fact that many probiotics and herbal medicines are targeted at the healthy

Marketing communication plan of MyBody Assignment

Marketing communication plan of MyBody - Assignment Example †¦Ã¢â‚¬ ¦..13 Bibliography †¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦Ã¢â‚¬ ¦15 Executive Summary Introduction MyBody is a growing force in high street premium beauty retail. With a network of 40 stores and growth plans over the next 18 months for a future of 20 more stores, this young and dynamic company is beginning to make its presence known. The purpose of this report is to provide a marketing communication plan for MyBody to show how it will continue to be sustainable in the future. Summary of Strategy and Aims of My Body We would like to create a strong market presence that will include our retail outlets and a stronger marketing presence on the Internet. We currently have 10% of our market and we would like to increase it to 20% which we believe can be done if we add the ability for people to order online. We believe that we can do this withi n the next 18 months if we add services to our website and if we increase our marketing efforts. MyBody Competitive and Brand Positioning Our biggest competitors are Penhaligens, PureBody and Boots. MyBody would be somewhere in the middle of these products because our Unique Selling Proposition would be that we are exclusively selling products for the body to make the skin healthier and the body more supple. We would expect that people who were concerned about their bodies would make a special effort to go to an expert in this field. We would be an expert because we offer these products exclusively and we do not offer other types of products like cosmetics, fragrances, or candles. Marketing and Strategic Objectives for 2011 The following marketing and strategic objectives are recommendations for 2011: 1. Create a stronger online presence using social networking to help existing and future customers know about our products. 2. To educate customers about the products they use and thei r affects on the body. 3. To promote the website online and in the local stores and encourage customers to order online. 4. To promote MyBody using FaceBook, Twitter and LinkedIN as sources for social networking support. 5. To create a series of articles about skincare for various trade magazines online and offline. Target Markets and Characteristics Our target markets are women who are baby boomers and who use the Internet and Generation Xers. Both of these have different characteristics that we must be aware of as we are marketing. As an example, Boomers are more interested in staying young and beautiful. Gen Xers want to feel that they are in control. Marketing Communication Plan for My Body Introduction MyBody is a growing force in high street premium beauty retail. With a

Iago, when No One is Looking Essay Example | Topics and Well Written Essays - 1000 words

Iago, when No One is Looking - Essay Example Iago's soliloquies and asides, in contrast to his speeches in the presence of other characters, reveals quite clearly his deceitfulness and self-disguise. The audience is not duped for a moment, as, through dramatic irony and our privileged position, we come see that he is engaged in "double-knavery" (1.2.318), and not at all the "honest and true" Iago that others believe him to be. The audience would be amazed at the absolute faith that the character have in Iago's honesty, which we know to be a lie even from his first appearance onstage. Iago's aim throughout the play is to "blacken" Othello's reputation to match the latter's face, in revenge for what he sees as a slight in Othello's appointing Cassio lieutenant over him. In his first soliloquy in Act I, scene iii, he gives three motives for his plotting: to fatten his purse (1.2.308), "to get [Cassio's] place" as lieutenant (1.2.318), and to make "sport" on Othello whom he hates (1.2.311). He later admits that he is somewhat in love with Desdemona, and reiterates his suspicion that "the lusty Moor/ Hath leap'd into [his] seat; the thought whereof/Doth gnaw at [his] inwards" (2.1.310-14), and that one of his motives is to be avenged "wife for wife" (2.1.315). Jealousy, susJealousy, suspicion and envy are not only key motives to Iago's actions, but are key elements of his character, as his soliloquies reveal. Not only does he harbor unfounded suspicions that Othello "hath done office 'twixt [his] sheets" (1.2.312-313), he suggests that Cassio also is likely to cuckold him (2.2.320). He gives a further reason for his jealousy of Cassio, who "hath a daily beauty in his life/That makes [him, Iago] ugly" (5.1.19-20). It is not surprising then, that the method he employs to embroil Othello in jealousy is based on the working of his own suspicious mind, where "mere suspicion /Will do as if for surety" (1.2.314-15). On the other hand, his soliloquies reveal that he is an astute observer of character. He knows people's nature well, and thus is able to use their weaknesses and foibles to his advantage. For example, he shows keen insight into the relationship between Othello and Desdemona, in particular the latter's influence on her husband. As a result, he plans to manipulate Desdemona's liberal tongue and goodness to "enmesh them all" (2.3.366) and "turn her virtue into pitch" (2.3.364). He is also aware of Othello's own insecurities, and inferiority complex due to his race. Despite the fact that Desdemona, "had eyes, and chose [him]", Othello is convinced that she is unfaithful and gives as a reason: Haply, for I am black And have not those soft parts of conversation That chamberers have, or for I am declined Into the vale of years (3.1.402-5), giving Iago fodder to work on his jealousy. Knowing that Cassio cannot hold drink, Iago manipulates him into drinking himself "full of quarrel and offence" (2.3.48), which action will lead him to a fight not becoming to his station, discrediting him in Othello's eyes, and allowing Iago to have the coveted position. He also reveals the means by which he will achieve his "monstrous" ends: by insinuating false motives to Cassio, who is handsome, charming, "framed to make women false" (1.2.323). Iago soliloquies further reveal his real thoughts about these

Radical Reconstruction Essay Example | Topics and Well Written Essays - 500 words

Radical Reconstruction - Essay Example Congress passed the Civil Rights Act of 1866, established the Freedmen’s Bureau intended to stop the lawmakers in the South from passing discriminatory legislation. President Johnson angered Radical Republicans by vetoing these laws but both were overridden by Congress which then rejected the Supreme Court decision in the Dred Scott case that denied citizenship rights to freed slaves by passing the 14th Amendment. In addition, it provides that no state can â€Å"deprive any person of life, liberty, or property, without due process of law.This Amendment also gave the right to black men aged 21 and over to vote and run for public office. However, despite these reconstructive legislative efforts white on black violence became increasingly commonplace in the South.Ex-Confederate troops and southern loyalists formed paramilitary organizations to fight the Union Army to kill all blacks and any whites that opposed their cause. White vigilante organizations derailed Reconstruction and re-established ‘home rule,’ a term used to describe whites only in the government and legal system. Blacks were terrorized and threatened not vote and certainly not run for office. The devastated South never received economic help that had been previously promised by Lincoln thus never fully recovered economically. The oppression of Blacks likely continued to a greater degree and for a longer period of time due to Lincoln’s assassination. The cumulative amount of human misery caused by Johnson is incalculable.

Organizational Conflict Essay Example for Free

Organizational Conflict Essay Conflicts within an organization can be difficult on employees. Some conflicts may be petty and some could end up in violence. They are often started because of the difference of opinions between employees (Shetach, A., 2012). Regardless of the situation; employers cannot afford to have conflicts within their organization. The purpose of this paper is to analyze the reason for the conflict within this organization and to come up with ways to help solve the conflicts. Description of the Conflict The conflict at my place of employment is that there are never enough associates scheduled to perform the duties that are needed to be done. The ICS team is the main team that goes through the inventory in the back room on a daily basis. Their job is to bin every item that comes within this store with a labeling system. The problem that is being cause is that there are not enough associates able to keep up with binning the items after the ICS team finishes scanning and counting their freight. What conflict that has occurred from this is that the unloaders are coming in and they have to help them out. In return the un-loaders are falling behind in their work. When the morning shift associates comes in; mainly department managers they have to work out the items that the ICS team has scanned. When you look at the amount of freight that is needing to be work out, it could be from four to fifteen L-carts. L-carts are the large carts that are used to move large freight to the floor or to the customer’s car. Production was falling behind because there was not a good plan set into place on who, what, and how the receiving area of the store would be operated. There was lack of communication that was going on between upper management and lower management. It seemed as though the associates were the ones that were making the decisions instead of the managers. The ICS team and the un-loaders were constantly arguing with each other, claiming that the other one is not doing their job. The department managers were coming in to work stating that the overnight associates were not putting out freight. It looked as if the same freight was left from the day before was not being worked. Level of Conflict There are four levels of conflict within an organization (Baack, D., 2012). What is going on within this organization is called intragroup conflicts. Intragroup conflicts occur when incidents between members of a team do not have the same mind set concerning goals. They disagree with the way the operations are being handled and the leadership (Baack, D., 2012). If conflicts are left unattended within an organization it could affect the overall goal of the company. The teams may not do what they need to do and the decision that they make in doing their job may not be the right decision (Baack, D., 2012). Propose Steps to Resolve the Conflict There are five steps that must be followed in order to help resolve conflicts within this organization. The first thing is to identify the parties that are involved in the conflict. Determining if the entire team is involved or if there is an individual that is causing the conflict need to be investigated first (Baack, D., 2012). By doing this, it will allow for the managers to help determine what is need to be done in order to resolve the conflict. This conflict can be stopped with the proper communication between team members and management. But they need to have weekly meeting in order to go over their goals and their concerns (Shetach, A., 2012). Having meeting will allow the team members to voice their opinions if they feel they may not be doing ample amount of production that is needed in order to be productive. This can be analyzed by management as soon as it is brought up in the meeting. The management team needs to find out what the real issue is within the team and figure o ut how the problem started. While they are investigating this issue they need to also know what position that each team member have concerning this conflict (Baack, D., 2012). This can help management to understand where each team member stands on this conflict so it could help them come up with a better solution in order to stop the problem. Finding an area in which there can be bargaining is very important in resolving conflicts within an organization (Baack, D., 2012). Management need to stay neutral in making a decision regarding the conflict that is going on between the ICS team, the unloaders, and the department managers. There should be lines in which the managers need to be able to listen to all sides and not show favoritism. Finding the best solution for the organization in what is important. The policies need to be addressed at the time when bargaining process is going on (Montgomery, M., 1995). At the same time, the team members need to be able to work through their conflicts. There need to be a check list that needs to be done on a daily basis in order for each backroom associate to follow. If they are not able to do what they are assigned then they should be able to type in the reason why. For example, I was called to work in the garden center by Assistant Tony. It should have the time, and date on the log so that it can show the reason why a task was not completed. Management should not be able to call an ICS member out of their work area for more than 15 minutes a day. Considering they might be one of four that was assigned to scan in what is coming off of the trucks. Another step that could help resolve the conflict is to schedule people with the department managers that only have one associate. Some department managers do not have more than one associate within th eir area. A department manage usually have five hundred to five thousand prices changes to do on a daily basis. They have to work their bins; bins are the freight that they have to put out on the shelves. If more associates are schedule past 5 pm, it could help the organization to make a better profit; because the shelves will be filled. Not all conflict situations are the same. Some may be resolved if the people that are involved learn the effect that their opinion, attitudes, or behavior have on other team members (Montgomery, M., 1995). Some of the managers need to be more productive by helping the team members to see what they need to improve in or what they have to offer to make the team stronger. Outcomes to the Conflict Resolution By not doing anything and letting the teams argue about what each other are doing it will cause a lack of production within the team. It could affect the entire team as a whole. Agreement is very important in a conflict resolution (Baack, D., 2012). When all of the team members on the ICS, department managers, and the un-loaders believe that their opinions matters that is when agreement is reached. The managers have to let them all know that all of the concerns that were leading up to their conflicts have been met. Everyone will be held accountable for their own job expectation, and no one will be pulled out of their area for more than fifteen minutes a day. The night managers will make sure that they have associates working in areas that have the largest amount of freight in order to keep the department managers from being over whelmed. Stronger relationships may develop between the teams; however, some teams like to work without other teams being involved. Take the ICS team for example; they do not trust anyone to count what is coming in on the truck. If an upper member of management scans what comes in on the truck they do not have much of a choice. If a new employee is in training they have to gain trust within this group before they let them work by themselves. And finally organizational learning can take place among team members. The can learn how to work together by coming up with better ways to find solutions to the problems they may encounter within their team (Baack, D., 2012). They can learn how to deal with each other on a personal level because they may have learned what each team needs. If the meetings continue to be given between these three teams on a weekly basis, this will teach them. In conclusion, there conflicts can make it difficult for team to work together. This paper was to show ways that teams could resolve their conflicts in order to help their organization to run smoothly and put a handle on conflicts. References Baack, D. (2012). Organizational behavior. San Diego, CA: Bridgepoint Education, Inc Montgomery, M., (1995). Five steps to resolves conflicts. Supervisory Management, 40(10), 8 Shetach, A., (2012). Conflict Leadership. Navigating Toward Effective and Efficient Team Outcomes. Journal for Quality Participation, 35(2), 25-30

Review of DNA and Protein Microarray for BioMEMS Technology

Review of DNA and Protein Microarray for BioMEMS Technology In recent years increase in genetically caused diseases is one of the major threat to mankind. Some of the genetically caused diseases are down syndrome, diabetes, obesity, sickle cell anemia, cystic fibrosis. This review paper explains how BioMEMS (Biological MicroElectroMechanicalSystem) technology used in microarrays and finding of gene expression which leads to medicine for particular diseases. BioMEMS research has been acquiring importance, due to the possibility of exploiting miniaturization to create new opportunities in medicine. BioMEMS systems in general have more diversity of materials and function than conventional MEMS devices. In BioMEMS ink-jet printing, photolithography techniques were introduced to deposit protein and DNA in array. DNA and protein micro-arrays based BioMEMS could be very extensively for rapid detection, drug discovery, and screening, especially when combined with integrated micro-fluidics and sensitive detection technologies. The techniques used to d efine patterns on semiconductor surfaces were utilized to construct arrays of single-stranded DNA. Once single strands of known sequences (capture probes) are placed at specific known sites on a chip surface, hybridization with molecules of unknown sequence (target probes) can reveal the sequence. Microarray-based gene expression profiling can be used to identify genes whose expression is changed in response to  disease caused genetically by comparing gene expression in infected to that in uninfected cells or tissues. Protein and antibody arrays can play a key role in search for disease-specific proteins that have medical, diagnostic, prognostic, and commercial potential as disease markers or as drug targets and for determination of predisposition to specific disease via genotypic screening. Array-based integrated chips and micro-fluidics hold a great potential for the development of high-throughput approaches to systematically analyze these proteins and to assign a biological fun ction, determine protein-protein and protein-DNA interactions. This paper tells about varies applications of BioMEMS to detect the defective gene the causes diseases and the fabrication methods used in microarrays chip production. Keywords: LOC Lab-on-a-chip, BioMEMS (Biological MicroElectroMechanicalSystem), ÃŽÂ ¼TAS (Micro Total Analysis System), Oligonucleotide, Microdroplets , Electrospray. 1. Introduction Microarray technology has been applied to study of gene expression to study mechanisms of diseases and to accelerate the drug discovery process. There is a definite trend towards increasing the use of molecular diagnostic methods, and biochip technologies, along with bioinformatics techniques. Classification of human disease using microarrays is considered to be important. The emphasis is not only on diagnosis but also on disease management, including monitoring the effect of treatment and determining prognosis [1]. Microarray and lab-on-a-chip systems are going to fulfill these new requirements, including the miniaturization of biological assays as well as the parallelization of analysis. Although the concept has been performed by miniaturizing the analytical equipments, the technology comes from the microeletromechanical and microelectronics industries [2]. Lab-on-a-chip technology is the method of choice to integrate processes and reaction and scale them down from conventional gla ssware to microfluidics, involving micro-sized channels in glass or polymer chips [3]. DNA microarray also knows as DNA chips, comprise a new technology emerging at a tremendous pace because of its power, flexibility, sensitivity and relative simplicity [4]. BioMEMS for proteomics can be divided into LOC device for specific tasks such as protein isolation, purification, digestion, and separation; and microarray device for high throughput study of protein abundance and function. An emergence of DNA, protein microarray has emerged over the last few years with commercial potential beyond the confines of the research laboratory [5]. In this paper we start our discussion with the history of microarray; subsequently we go into the details of general techniques used in DNA and protein microarray followed by fabrication and the application and future of microarray. 2. History of Microarray Microarray technology evolved from Southern blotting, where fragmented DNA is attached to a substrate and then probed with a known gene or fragment [6]. The first reported use of this approach was the analysis of 378 arrayed lysed bacterial colonies each harboring a different sequence which were assayed in multiple replicas for expression of the genes in multiple normal and tumor tissue [7]. These early gene arrays were made by spotting cDNA onto filter paper with a pin-spotting device. The use of miniaturized microarray for gene expression profiling was first reported in 1995 [8]. This technology allowed scientists to analyze thousands of mRNAs in a single experiment to determine whether expression is different in disease state. Unfortunately, mRNA levels within a cell are often poorly correlated with actual protein abundance [9]. A complete eukaryotic genome on a microarray was published in 1997[10]. The development of biochip has a long history, starting with early work on the und erlying sensor technology. In 1953, Watson and Crick announced their discovery of now familiar double helix structure and sequencing techniques by Gilbert and Sanger in 1977 [11, 12]. Two additional developments enable the technology used in modern DNA-based biosensors. First, in 1983 Kary Mullis invented the polymerase chain reaction (PCR) technique, a method for amplifying DNA concentration. This discovery made possible the detection of extremely small quantities of DNA in samples. Second, in 1986 Hood and co-workers devised a method to label DNA molecules with fluorescent tags instead of radiolables, thus enabling hybridization experiments to be observed optically [13]. A big boost in research and commercial interest came in the mid 1990s, when ÃŽÂ ¼TAS (Micro Total Analysis System) technology turned out to provide interesting tooling for genomics application, like capillary electrophoresis and DNA microarray [14]. Immunoassays, the precursor to protein chips available since t he 1980s, exploit the interactions between antibodies and antigens in order to detect their concentrations in biology sample. Their creation, however, is tedious and expensive. As to this, research at Harvard University combined the technology of immunoassays and DNA microarray to develop the protein chip [15]. 3. DNA Microarrays and Fabrication 3.1 Introduction Microarray analysis allows simultaneous of gene and gene products, including DNA, mRNA and proteins. There are basically two formats: cDNA microarrays and oligonucleotide microarrays. A cDNA microarray is an orderly arrangement of DNA probe spot printed onto a solid matrix such as glass, nylon, or silicon. The substrate is usually less than 4ÃÆ'-4 cm, while the spot size is less than 250ÃŽÂ ¼m. A DNA molecular probe is tethered (embedded and immobilized) to each spot on microarray. surface modification of the substrate, such as wit poly-L-lysin or silane, facilitates adhesion of the DNA probes. Hybridization is the base pairing between target and the probe, and is limited by the sensitivity and specificity of the microarray. There are three basic types of oligonucleotide microarrays: gene expression, genotyping (SNPs), and resquencing. Genomic DNA may be used for the study of SNPs, while expressed DNA sequence (cDNA clones, expressed sequence tags or ESTs) are used for gene expre ssion [17]. 3.2 Microarrays for Gene Expression Gene expression microarrays are tools that tell how much RNA (if any) a gene is making. Since 1977, and prior to microarray, only a few genes could be studied at a time using the northern blot analysis. GeneChip (Fig. 1.1) microarrays use the natural chemical attraction, or hybridization, between DNA on the array and RNA target molecule from the sample based on complementary base pairs. Only RNA target molecule that have exact complementary base pair bind to the prob. Gene expression detection microarray is that they are able to measure tens of thousands of genes at a time, and it is this quantitative change in the scale of gene measurement that has led to a qualitative change in our ability to understand regulatory processes that occur at the cellular level. It is possible to obtain near comprehensive expression data for individual tissues or organs in various states. Compressions are possible for transcriptional activity across different tissue, and group of patients with and witho ut a particular disease or with two different diseases. Microarray studies are designed in principle to directly measure the activity of the genes involved in particular mechanism or system rather than their association with a particular biological or clinical feature [18]. Although genes may be thousand of base pairs long, it is only necessary to construct a probe of 25 bases that represent a unique complementary portion of the target gene. In other words, the short probe on the microarray measures the expression of the complete gene by sampling only a small section of the gene. In some instances, as little as one RNA molecule out of 100,000 different RNAs in an original sample may be detected [19]. Sensitivity is the ability to identify the rarely expressed transcripts in a complex background. Specification is the ability to discern between different family members. The hybridization efficiency of two nucleic acid strand depends on 1) Sequence-dependent factors for length, extent of complementarity, and overall base composition; 2) Sequence independent factors such as the concentration of the probe and target, time, temperature, cation concentration, valency character, pH, dielectric and chaotropic medica, surface characteristics of the solid, and density spacing of the probe molecules; and 3) Sample-dependent complex background signal, which are probes interacting with the wrong complementary sequence [20]. Fig 1.1 GeneChip probe microarray cartridge (Image courtesy of Affmetrix) 3.3 Microarray for SNPs Small difference in a DNA sequence can have major impact on health. Deletions, insertions, and other mutations of as little as a single base pair may result in signification disease. Identification these mutations require determining the exact sequence for thousand of SNPs distributed throughout the genome. Using microarray, it is possible to scan the whole genome and look for genetic similarities among a group of people who share the same disease. Using microarray to genotype 10,000 to 100,000 SNPs, it is possible to identify the gene or group of genes that contribute to disease. For example, if a large group of people with a given diagnosis have several SNPs in common, but not healthy people, then mutations may be looked for within those SNPs. A genotyping microarray may look for up to 100,000 SNPs or more [21]. 3.4 Fabrication DNA spotting may be accomplished by depositing PCR amplified ESTs (500-5000 base pairs), or by in suit synthesis of oligodeoxynucleotide sequences (20-50 base pairs) on the substrate. There are variety of spotting techniques that include mechanical and ink-jet style application. The GeneChip brand arrays provide high levels of reproducibility, sensitivity, and specification. The following process steps are used for fabrication of the GeneChip: 1) GeneChip probe array are manufactured through a combination of photolithography (Fig 1.2) and combinatorial chemistry. With a calculated minimum number of synthesis steps, GeneChip technology produce array with hundreds of thousands of different probes packed at an extremely high density. Small sample volumes are required for study. Manufacture is scalable because the length of the probe, not their number, determines the number of synthesis steps required. 2) Manufacturing begins with a 5-in square quartz wafer. Initially the quartz is washed to ensure uniform hydroxylation across its surface. Because quarts is naturally hydroxylated, it provides an excellent substrate for the attachment of chemical, such as linker molecules, that are later used to position the probes on the arrays. Fig 1.2 Photolithographic technique are used to locate and add nucleotides for fabrication of array of probe (Image courtesy of Affymetrix) 3) The wafer is placed in a bath of silane, which reacts with hydroxyl groups of quartz, and forms a matrix of covalently linked molecules. This distance between these silane determines the probes packing density, allowing array to hold over 500,000 probe location, or features, within a mere 1.28cm2. Each of these features harbors millions of identical DNA molecules. The silane film provides a uniform hydroxyl density to initiate probe assembly. Linker molecules, attached to the silane matrix, provide a surface that may be spatially activated by light (Fig 1.3). 4) Probe synthesis occurs in parallel, resulting in the addition of an A, C, T or G nucleotide to multiple growing chains simulataneously. To define which oligonucleotide chains will receive a nucleotide in each step, photolithographic masks, carrying 18 to 20 ÃŽÂ ¼m2 windows that corresponds to the dimensions of individual features, are placed over the coated wafer. The windows are distributed over the mask based on the desired sequence each. When the UV light is shone over the mask in the first step of synthesis, the exposed linkers become deprotected and are available for nucleotide coupling. critical to this step is the precise alignment of the mask with the wafer before each synthesis step. To ensure that this critical step is accurately completed, chrome marks on the wafer and on the mask are perfectly aligned. 5) Once the desired features have been activated, a solution containing a single type of deoxynucleotide with a removable protection group is flushed over the wafers surface. The nucleotide attaches to the activated linkers, initiating the synthesis process. 6) Although the process is highly efficient, some activated molecules fail to attach the new nucleotide. To prevent these outliers from becoming probes with missing nucleotides, a capping step is used to truncate them. In additional, the side chains of the nucleotides are protected to prevent the formation of branched oligonucleotides. Fig 1.3 GeneChip fabrication steps (Image courtesy Affmetrix). 7) In the next synthesis step, another mask is placed over the wafer to allow the next round of deprotection and coupling. The process is repeated until the probes reach their full length, usually 25 nucleotides. 8) Although each position in the sequence of an oligonucleotide can be occupied by one of four nucleotides, resulting in an apparent need for 24ÃÆ'-4, or 100, different masks per wafer, the synthesis process can be designed to significantly reduce this requirement. Algorithms that help minimize mask usage calculate how to best coordinate probe growth by adjusting synthesis rates of individual probes and identifying situations when the same mask can be multiple times. 9) Once the synthesis is completed, the wafer are deprotected and diced, and the resulting individual arrays are picked and packed in flowcell cartridges. Depending on the number of probe features per array, a single wafer can yield between 49 and 400 arrays. 10) The manufacturing process ends with a comprehensive series of quality control tests. Additional, a sampling of array from every wafer is used to test the batch by running control hybridizations. A quantitative test of hybridization is also performed using standardized control probes [22]. 3.5 Microarray Data Analysis Data filtration is performed by selecting threshold pixel intensity; and 2-, 5-, or 10- fold difference between the samples. Different genes with an identical profile may represent a coordinate response to a stimulus. Genes with opposite profiles may represent repression. To compare expression profiles it is necessary to define a set of metrics, or operations that return a value that is proportional in some way to the similarities or difference between two expression profiles. The most commonly used metrics are Euclidean distance and Pearson coefficient of correlation [23]. 3.5.1 Euclidean Distance Two or more profile of each of two genes are compared as a mathematical matrix operation of n-dimensional space, where n is the number of expression patterns available. The Euclidean distance is the square root of the summation of the difference between all pairs of corresponding values. For two genes the distance is as follows: Where d is the distance, e1 is the expression pattern of gene1, e2 is the expression pattern of gene 2, and i is the element of the expression profile: Gene1 (e11, e12, ., e1n) and gene1 (e21, e22, à ¢Ã¢â€š ¬Ã‚ ¦.,e2n). 3.5.2 Pearson Correlation Coefficient The Pearson correlation coefficient (r) gives a value of from -1 to 1, and closer to 1 (negative and positive correlation, respectively). The closer two profiles have the same expression, the closer the value will be to 1: Where and Sen are the mean and typical deviation of all of the point of the nth profile, respectively. 4. Protein Microarray and Fabrication 4.1 Introduction Protein microarrays are becoming an important tool in proteomics, drug discovery programs, and diagnostics [24]. The amount of information obtained from small quantities of biological samples is significantly increased in the microarray format. This feature is extremely valuable in protein profiling, where samples are often limited in supply and unlike DNA, cannot be amplified [25]. Protein microarrays are more challenging to prepare than are DNA chips [26] because several technical hurdles hamper their application. The surfaces typically used with DNA are not easily adaptable to proteins, owing to the biophysical differences between the two classes of bioanalytes [27]. Arrayed protein must be immobilized in a native conformation to maintain their biological function. Unfortunately, proteins tend to unfold when immobilized onto a support so as to allow internal hydrophobic side chains to from hydrophobic bonds with the solid surface [28]. Surface chemistry, capture agents, and detect ion methods take on special significance in developing microarrays. Microarrays consist of microscopic target spots, planer substrates, rows and columns of elements, and probe molecules in solution. Each protein assessed by a microarray should be the same as the partial concentration of each protein in the biological extract [29]. The past ten years have witnessed a fascinating growth in the field of large-scale and high-throughput biology, resulting in a new era of technology development and the collection and analysis of information. The challenges ahead are to elucidate the function of every encoded gene and protein in an organism and to understand the basic cellular events mediating complex processes and those causing diseases [30-33]. Protein are more challenging to prepare for the microarray format than DNA, and protein functionality is often dependent on the state of proteins, such as post-translational modification, partnership with other proteins, protein subcellular locali zation, and reversible covalent modification (e.g. phosphorylation). Nonetheless, in recent years there have been considerable achievements in preparing microarray containing over 100 proteins and even an entire proteome [34-36]. Randox Laboratories Ltd. Launched Evidence, the first protein Biochip Array Technology analyzer in 2003. In protein Biochip Array Technology, the biochip replaces the ELISA (Enzyme-linked immunosorbent assay) plate or cuvette as the reaction platform. The biochip is used to simultaneously analyze a panel of related tests in a single sample, producing a patient profile. The patient profile can be used in disease screening, diagnosis, monitoring disease progression or monitoring treatment (wiki Biochip). Protein expression profiling, protein-protein binding, drug interaction, protein folding, substrate specificity, enzymatic activity, and the interaction between protein and nucleic acids are among the application of protein microarrays. Abundance-based microarray, including capture microarray and reverse-phase protein blots, measure the abundance of specific biomolecules using well defined and high specific analyte-specific reagents (ASRs). Different classes of molecules can act as capture molecules in microarray assays, including antigen-antibody, protein -protein, aptamer-ligand, enzyme-substrate, and receptor-ligand [37]. 4.2 Spotting In situ synthesis of protein microarrays as done for DNA microarrays is impractical. Other forms of delivery-based technology must be incorporated. One-drop-at-a-time (microspotting) techniques including use of pins, quills or hollow needles that repeatedly touch the substrate surface depositing one spot after the next in an array format; shooting microdroplets from a ejector similar to ink-jet printing; and depositing charged submicron-sized droplets by electrospray deposition (ESD). Alternatively, parallel techniques such as microcontact printing (ÃŽÂ ¼CP), digital ESD, and photolithographic controlled protein adsorption can be used. Currently, micospotting by robotic techniques has greater use in the research setting, whereas parallel techniques offer cost saving for mass production for commercial use [38]. 4.3 Microcontact printing (ÃŽÂ ¼CP) In microcontact printing stamps are typically made from a silicon elastomer and used to make a microarray of spots with feature size from 0.01 to 0.1ÃŽÂ ¼m. Steps for stamping include the following [38]: 1) Activation of the stamp surface to increase hydrophilicity or to introduce grups for inking to target molecules such as antibodies, protein A, or streptavidin. 2) Direct adsorption of protein molecules or their binding to capture molecules over a period of 0.5-1 hours. 3) Rinsing. 4) Drying in a nitrogen stream for about a minute. 5) Pressing the stamp against a suitable substrate for about a minute to allow transfer of the semidry materials. Disadvantages include poor control of the amount of materials transferred, small amount of deposited materials, and possible changes in protein function. Microarrays containing up three different proteins were fabricated by ÃŽÂ ¼CP technique and tested as a detection system for specific antibodies [39]. Immunoassay were successfully performed using the patterned protein microarrays, and were characterized by fluorescence microscopy and scanning- probe microscopy. The characterization revealed the quality of the protein deposition and indicated a high degree of selectivity for the targeted antigen-antibody interaction. 4.3 Electrospray Deposition (ESD) The basic physics underlying the newly emerging technique of electrospray deposition (ESD) as applied to biological macromolecules. Fabrication of protein films and microarrays are considered as the most important applications of this technology. All the major stages in the ESD process (solution electrification, formation of a cloud of charged microdroplets, transformation of microdroplets into ions and charged clusters, deposition, and neutralization) are discussed to reveal the physical processes involved, such as space charge effects, dissipation of energy upon landing and neutralization mechanisms [40]. In electrospray deposition, protein is transferred from the glass capillary positioned 130-350 ÃŽÂ ¼m above a conducting surface. Micro-sized charged droplets move in an electric field created by the difference in electric field potential between the tip and the substrate surface and by the spatial charge of the droplet cloud. The electrostatic repulsion expands the cloud, and microdroplets are deposited as a round spot. The spot density is greater at the center [38]. Two new techniques were recently developed in these laboratories for fabrication of protein microarrays: electrospray deposition of dry proteins and covalent linking of proteins from dry deposits to a dextran-grafted surface. Here we apply these techniques to simultaneously fabricate 1200 identical microarrays. Each microarray, 0.6 ÃÆ'- 0.6 mm2 in size, consists of 28 different protein antigens and allergens deposited as spots, 30à ¢Ã‹â€ Ã¢â‚¬â„¢40 ÃŽÂ ¼m in diameter. Electrospray deposition (ESD) of dry protein and covalent linking of proteins from dry deposits to a dextran-grafted surface has been studied from fabrication of microarrays. Electrospray (ES) deposition has been applied to fabricate protein microarrays for immunochemical assay. Protein antigens were deposited as arrays of dry spots on a surface of aluminized plastic. Deposition was performed from water solutions containing a 10-fold (w/w of dry protein) excess of sucrose. Upon contact with humid air, the spots tur n into microdroplets of sucrose/protein solution from which proteins were either adsorbed or covalently linked to clean or modified aluminum surfaces. It was found that covalent binding of antigens via aldehyde groups of oxidized branched dextran followed by reduction of the Schiff bonds gives the highest sensitivity and the lowest background in microarray-based ELISA, as compared to other tested methods of antigen immobilization [41]. Protein microarray with an antibody-based protein array for high-throughput immunoassay, with an ESD method using a quartz mask with holes made by an abrasive jet technique, has been performed. An antibody solution was electrosprayed onto an ITO glass, and then antibodies were deposited and cross-linked with a vapor of glutaraldehyde. The dimeters of the spots were approximately 150 ÃŽÂ ¼m. The arrays were then incubated with corresponding target antigenic molecules and washed. The captured antigens were collectively detected by fluorescence and chemiluminescence. The signals were quantitatively visualized with a high-resolution CCD [42]. 4.4 Surface immobilization In many proteomics applications, one is interested in the facile and covalent immobilization of protein molecules without the use of any special tag or chemical modification. This is most conveniently achieved via chemical reactivity towards the commonly available -NH2 groups on the surface of protein molecules. One of the most efficient leaving groups towards -NH2 is N-hydroxysuccinimide (NHS) attached via an ester bond. We have developed an NHS surface based on the zero background PEG coating. It allows for fast immobilization reactions with the remaining NHS groups easily washed off to expose the zero background PEG coating (Fig 1.4). In subsequent assays, the PEG functionality ensures that binding of particular molecules to the surface is only through the specific interaction with the immobilized protein molecule and the commonly seen background problem is solved without the need of a blocking step. Fig 1.4 NHS activated surfaces for the immobilization of proteins, peptides, antibodies (Image courtesy: ZeroBkg ® ) Peptide and protein microarrays fabricated on NHS/PEG/glass slides (Fig 1.5) Nanoliter droplets of peptide (21 amino-acids) or protein (fibrinogen) solution containing 10% glycerol are deposited on the glass slide with a robotic arrayer and incubated for 10 minutes. NHS-groups in remaining area are removed by a deactivating buffer for 30 minutes at room temperature. The immobilized peptide or protein on the surface is detected by incubation with the primary antibody specifically against the peptide or fibrinogen, followed by wash and incubation with cy3-conjugated secondary antibody. The glass slides are imaged on a laser scanner. The most important result is the exceptionally low background due to the PEG coating. While the NHS/PEG coated glass slides are ideal for protein, peptide, and antibody arrays, they are also useful as low background surfaces for other microarrays, such as oligonucleotides, carbohydrates, and other small molecules. The non-fouling property of the high densit y PEG coating becomes critically important when one uses such an array for the study of complex biological samples, such as plasma or serum. In order to detect molecules of low abundance, such as cancer biomarkers, one needs to minimize non-specific adsorption of other abundant biomolecules [43]. Fig 1.5 Fluorescence images of peptide (left) and protein (Fibrinogen, right) microarrays fabricated on NHS/PEG/glass slides and detected by immunostaining. The diameter of each spot is ~100 ÃŽÂ ¼m (Image courtesy: ZeroBkg ® ).   4.5 Self-assembling Protein Microarrays Molecular fabrication of SAMS depends on chemical complementarily and structural compatibility, both of which confer the weak and noncovalent interaction that bind building blocks together during self-assembly. Water-mediated hydrogen bonds are important for living system. In nature the assembly of peptide and proteins has yielded collagen, keratin, pearl, shell, coral and calcite microlenses, and optical waveguides [44]. The application of self-assembly techniques in the design of biocompatible protein microarray surfaces, immobilizing cells, and lipid layers, and spotting techniques has been reviewed by others [45-46]. 4.6 Detection Strategies Detection and readout of complex formation in each spot is performed with fluorescence, chemiluminescence, mass spectrometry, radioactivity, or electrochemistry. Label-free methods include mass spectrometry and SPR. Labeled probe methods include use of a chromogen, fluorophor, or a radioactive isotope. Direct strategies use a labeled antibody to directly bind to the target molecule immobilized on the substrate. Amplification strategies based on avidin-biotin binding enhance sensitivity. Indirect strategies use an immobilized antibody for capturing labeled, specific molecules from the sample. Sandwich assay as noted earlier require two distinct antibodies foe detection of a capture molecule. The first antibody is immobilized on the substratum, and serves to capture the molecule of interest. A second labeled antibody then binds to the first complex allowing detection [47]. 5. Application of Microarray Ever since the first 1000 probe DNA microarray was reported over a decade ago [48], great strides have been made in both quantitative and qualitative applications. Today, a standard DNA chip contains up to 6.5 million spots and can encompass entire eukaryotic genomes. A plethora of alternative applications are continually reported, albeit at various stages of maturity. What was once seen solely as a transcript profiling technology has now emerged as a reliable format for genotyping, splice variant analysis, exon identification, ChIP-on-chip, comparative genomic hybridization (CGH), resequencing, gene synthesis, RNA/RNAi synthesis and onchip translation [49]. Perhaps the most exciting recent developments from a drug discovery perspective come from the integration of diverse technological innovations into microarray-based solutions, especially for other classes of molecular entity. From small molecules (e.g. metabolites, nucleotides, amino acids, sugars) to oligomeric and polymeric der ivatives thereof, microarrays are now allowing us to examine the intra-class (e.g. protein-protein) and inter-class (e.g. protein: small molecule) interactions of these bio-system components on a systems-wide level. Yet, despite the appearance of a diversity of microarray types (e.g. Small Molecule Microarrays (SMMs) [51], Protein-Nucleic acid (PNA) microarrays [52], Glyco-chips [53], peptide chips [54], antibody chips [55], cell and tissue microarrays [56]), each differs in their relative contribution to the Voltaire challenge. Certainly the foremost of such opportunities are thos

Conflict Resolution And Transformation

Conflict Resolution And Transformation Conflict resolution and transformation are issues which have become very topical in debates and discussions on Zimbabwe. This is not only because Zimbabwe is characterised by many conflicts, but much more so due to the realization that in most cases the conflicts have negative impacts the a nations socio-economic and political development. Thus conflict resolution and peace building processes have become very essential in solving the problem of conflicts in the country. This chapter intends to examine the grassroots conflict resolution and peace building processes in Zimbabwe with a focus on Tongogara district as case study. It will also seek to explore the context, in which traditional leaders operate, the processes that link them to the people as well as how other scholars view them in terms of their effectiveness. Saunders (2000) defined literature review as a search and evaluation of the available literature in a given subject or area. Reviewing related literature helps the researcher gain insight on what other researchers have done and establishes existing gaps which the research seeks to ultimately fill. Punch (1998) considers the review of related literature as a researchers roadmap in the quest to convert tentative research problems to a detailed and concise plan of action. Lincoln (2005) augments the same notion by suggesting that it is of importance to be guided by related literature as this will equip the researcher with requisite skills to evaluate various viewpoints basing on the work that other researchers have done. The literature reviewed in this chapter seeks to analyse the role of traditional leaders in conflict transformation, the concept of conflict transformation, the common conflicts experienced at grassroots level and more importantly the approaches that are used by the traditional authorities in transforming conflicts. It further seeks to examine the theoretical and scholarly perspectives on the effectiveness of community processes that are employed in conflict management and peace building. 2.1 Background to conflict theory The period from 1970 to the present, has witnessed a remarkable interest in studies in conflict management and transformation. This was motivated by a number of factors which include ideological changes in the international system, the independence of most African states and the rise of many civil wars in Africa and the rise of new actors in conflict resolution paradigm. Interestingly however, most of the literature produced focuses mainly on the documentation of conflicts, their nature, and types of resolution that can be achieved neglecting the area of conflict prevention, resolution, transformation and management; hence the need to research on the practical ways that can help in the achievement of sustainable peace especially at grassroot level. Gaps have also been noticed in terms of the level at which conflict analysis is taking place as many scholars tend to place conflict resolution and transformation at the high level of governance not considering that many causes of conflict are deeply rooted in the grassroots and require the grassroots level actors to act on them to achieve sustainable peace and development. Sandy (2004) places emphasis on the conditions that are necessary for the transformation of conflicts. He argues that any attempt to articulate the nature of conflict and conflict resolution, must address those conditions, which are favourable for its emergence. He mentioned participation, engagement, freedom, justice and human rights as pre requisites for the achievement of conflict transformation. Sandy (2004) also mentioned the need for Community building and democratization as important strategies in conflict resolution and transformation .However Sandy does not unpack on how the democratization and community building can be used to achieve conflict transformation at the lowest level of governance where there are traditional leaders and councillors as key actors in governance. This therefore leaves out players like the grass root leaders and other influential people in the communities who are also important in peace building. The Online Journal of Peace and Conflict Resolution (2 010) focuses on definitions of peace and conflict, bringing the different kinds of peace that are there and the pre-requisites for conflict transformation and peace , the mechanisms of achieving this peace is however often overlooked. This therefore leaves a gap in terms of literature that explores practical ways of resolving conflicts and peace building, Rumel (2004) looks at alternative concepts of conflict resolution and the principles underlying those concepts. He mentions peace, as being a state of mind, that is, if the mind is at rest, then it follows that there will be peace and vice versa. Rumel (2004) views the human mind, as major contributor to conflict as he points out that, for as long as one has unsatisfied desires he/she wont be at harmony with the others. He brings out an important aspect in conflict transformation when he states that a human being needs to be satisfied with oneself and the outcome of any resolution exercise that maybe employed. Harris and Reilly (2005), emphasize the need to build a strong grassroots background as critical in addressing deeply rooted conflicts. They emphasise on the importance of addressing the root causes of conflicts through the involvement of parties involved in the conflicts. Their focus is however more inclined to ethnic conflicts and tribal conflicts, as they believe these conflicts are more a result of identity than anything else. This work is important, as it looks some of the practical ways in conflict resolution that are needed to achieve peace more so at grassroots level as most ethnic and tribal conflict are noticed at lower levels of administration like the districts and the villages. Most of the literature on conflict turns a blind eye on the role of grassroots actors in conflict transformation as usually their focus is large scale level, this often does not bring sustainability and progressive development, moreover this also leads to the extinction or decline in relevance of the grassroots approaches that are being used in the rural communities in developing countries. 2.2 The concept of Conflict transformation Conflict transformation is the term that has come into common usage over the years from the early 1980s, as a concept and a process that encompasses various aspects of conflict prevention, peace building, supporting local capacities for peace and transformational development. Conflict transformation arose as an alternative to the dominant paradigms of conflict resolution. As advocated by Lederach (1995), conflict transformation was conceptualized to provide a comprehensive framework for addressing conflict throughout its phases, that is from the initial stages of indirect conflict, to full-scale direct conflict to lastly, its resolution. Conflict transformation seeks to address questions often neglected on conflict resolution; structural violence, culture and cultural identity and the role individuals can play in diminishing conflict intensity and duration (Miall et al 1999). However, conflict transformation has also been articulated as an extension of current practices incorporated in peace-building (Miall et al 1999). This distinction does not however, affect the primary goals and objectives of a transformational approach. A number of conflicts theorists like Lederach (1998) advocate for the pursuit of conflict transformation as opposed to conflict resolution and conflict management, this according to Lederach (2000) is because conflict transformation reflects a better understanding of the nature of conflict itself. Conflict resolution implies that conflict is bad hence something bad should be ended thus conflict can be resolved permanently though mediation and other intervention processes, conflict management on the other hand correctly assumes that conflicts are long term processes that people can be directed or controlled as though they were physical objects (Lederach 2000). Furthermore Conflict transformation as put by Lederach does not suggest that we simply eliminate or control conflicts but rather work with its dialectic nature, this takes into cognisance the fact that conflict is social and naturally created by humans who are involved in relationships, it changes (transforms) these events, peop le, relationships that created the initial conflict. The cause and effect relationships thus goes both ways from the people and relationships to the conflict and then back to the people and the relationships (Vayrnen 1991), thus in this sense conflict transformation describes a natural occurrence. Conflict Transformation also involves transforming the way conflicts are expressed, it may be expressed competitively, aggressively or violently or it may be expressed through nonviolent advocacy, coalition or attempted cooperation (Lederach 1998) .The Centre for Conflict Dynamics(CCD 2009) views conflict transformation as a process by which conflicts such as ethnic conflicts are transformed into peaceful outcomes , it is therefore a process of engaging with and transforming the relationship, interests, discourses and if necessary the very constitution of society that support the continuation of violent conflict. In support of this view by CCD (2009), The Search for Common Ground (SFCG 2010) sees conflict transformation as initiatives that are often characterised by long time horizons and interventions at multiple levels, aimed at changing perceptions and improving relationships and addressing the roots of the conflict including inequality and social justice. Conflict transformation theory recognises the need to transform the conflict at a number of levels. Vayrnen (1991) identifies five transformations that need to occur in order to have a positive shift in the conflict. These are context transformation, structural transformation, actor transformation, issue transformation and individual / group transformation. This approach to conflict transformation acknowledges the multi-dimensional nature of conflict. 2.2.1 Principles of conflict transformation Burton 1996 identifies several principles to which he argues form the backbone of a conflict transformation process. Conflict should not be regarded as an isolated event that can be resolved or managed but as an integral part of societys on-going evolution and development. Conflicts should not be understood sorely as inherently negative and destructive occurrence but rather as a potentially positive and productive force of change if harnessed constructively. Conflict transformation goes beyond merely seeking to contain and manage conflict, instead seeking to transform root causes of a particular conflict. Conflict transformation is a long term gradual and complex process requiring sustained engagement and interaction. Conflict transformation is not just an approach and set of techniques but a way of thinking about and understanding conflict itself. Conflict transformation is particularly intended for intractable conflicts, with deep rooted issues. 2.3 Background to Traditional Leadership in Zimbabwe Ranger (1996) poses that the institution of traditional leadership has been around in Africa from time immemorial, traditional leaders are according to ranger the politicians of the pre-colonial age. At independence in 1980, Chieftainship was retained as a symbol of traditional values but the chiefs themselves were stripped of all their administrative and judicial functions. The chiefs and headman even lost their tax collecting functions as well as some administrative customary functions. District Councils assumed the administrative functions previously performed by traditional rulers whilst community courts took over the judicial functions. Ncube (2011) posts that the failure by the new government to incorporate and co-opt traditional institutions into formal state institutions in the first decades of independence lies at the heart of the confusion surrounding local administration in the communal areas after independence, this confusion was characterised by lack of clarity on the roles and functions between the Traditional institutions of Chiefs, Headman and Village Heads and the elected leadership of Village Development Committees (VIDCOs) and Ward Development Committees WADCOs in land matters. This precipitated a crisis of communal leadership in the communal areas of Zimbabwe whereby the legitimacy of the traditional institutions began to be questioned .Ncube (2011) further states that the powers of the traditional leaders were becoming defunct in many areas of the country, some chiefs, headman and village heads required some of their defunct authority over land proceeded to clandestinely allocate land, this land allo cation has become the common source of conflicts in Zimbabwes rural areas, thus the crisis of communal leadership sufficed itself in many land conflicts which occurred throughout the country The Zimbabwe governance system like in most African countries is characterised by co-existence of hereditary chieftainship and a democratically elected leadership. Traditional leadership is active at all levels of governance in Zimbabwe from the national level to the village level. At the highest level of the institution is the Chief, at the middle of the hierarchy is the office of the Headman and at the lowest tier which is village level is the Village Head , These institutions are established by the Traditional Leaders Act which recognises the role of each office in community development and peace building. The institution of traditional leadership is also recognised by the constitution, unlike local government that is created by statutes of Parliament. There are however conflicting claims to legitimacy and uneasy co-existence between traditional and elected leadership. Traditional leadership and local government officials occasionally trade accusations of abuse of power, non-compl iance with laws; customs and traditions, especially regarding allocation and management of resources such as land which forms the prevalent source of most conflicts in communal areas The Headman has functions similar to those of the Chief on a delegated basis but he is also the chair of Ward Assembly meetings. Since the Village Head chairs both the VIDCO and Village Assembly, the VIDCOs survive on the hard work of the Village Head and in a number of cases the VIDCOs no longer operating with all VIDCO functions being performed by the Village Head whilst in some cases VIDCOs are only seen to be operating when land disputes and resource conflicts emanate (Moyo, 2006) 2.3.2 The rural Governance nucleus The above diagram gives an explanation on the relationship in rural governance. The people form the core of governance and everything that is done at local level. The Africa Community Publishing and Development Trust (ACPDT 2010) explains that the authority in the rural areas rise with rank from the ordinary citizen or resident of a local area until it reaches the level of the rural district council. Which form the overall leadership in terms of development policies in Zimbabwean rural areas? the powers of the village heads are less than that of the headman as given in the traditional leaders Act cap 29.17(111) section 11, whilst those of the Headman are more than those of the Village heads but less than those of the chief in terms of the same Act (section 6 and 8; Traditional Leaders Act). Matibenga (2010) Asserts that in conflict resolution, if a village head fails to solve a community conflict issue, they refer it to the headman and if a headman fails again that same issue is referred to the Chief, the Chief is the highest traditional authority in rural areas. Ncube (2011) however argues on the same line but stating differently that these hierarchies in the rural areas are the primary causes of conflicts in rural Zimbabwe, this is because there are conflicts between these traditional leaders themselves in terms of responsibilities as one can easily see that the Chief has no limits over his jurisdiction in the discharge of traditional authority, The chief has the power according to the Traditional Leaders Act and the Customary Law and Local Courts Act to deal with issues even those that can be adjudicated by the lower authority of traditional leadership as such this causes dissatisfaction amongst the headman and village heads, as a result if the lower authority try to adjudicated the same type of conflicts in future, their authority is easily undermined and as such this reduces their relevance in dealing with traditional matters. This duplication of duties has been going on well for quite some time and has thus undermined the co-existence of these traditional leaders and as such reduced the impact of the grassroots approaches to conflict transformation by the traditional authorities. 2.4 Aims of grassroots conflict resolution 2.4.1 Empowering the community Kubasu (2008) observes that grassroots approaches to conflict resolution by traditional leaders seek to re-empower communities to make vital decisions and address the needs of their people as well as create an environment more conducive to lasting peace. This is because empowering the traditional leader is empowering the community as the community banks on its leadership for progress, opinion and development. 2.4.2 Restoration of Order and Relationships From a traditional point of view, conflict is perceived as an unwelcome disturbance of the relationships within the community. Hence traditional conflict transformation aims at the restoration of order and harmony of the community. Cooperation between conflict parties in the future has to be guaranteed. Traditional conflict management is thus geared towards the future (Mare. 2004). Consequently, the issue is not punishment of perpetrators for deeds done in the past, but restitution as a basis for reconciliation. Reconciliation is necessary for the restoration of social harmony of the community in general and of social relationships between conflict parties in particular. The aim as put by Kubasu (2008) is not to punish, an action which would be viewed as harming the group a second time. The ultimate aim of conflict transformation thus is the restoration of relationships Another immediate objective of such conflict resolution is to mend the broken or damaged relationship, and rectify wrongs, and restore justice (Moyo 2009). This is to ensure the full integration of parties into their societies again (Bob-Manuel: 2006), and to adopt the mood of co-operation for progressive development. 2.4.3 Transforming societies Other long term aims are based on building harmony in the community. It has been realised that tolerance is not maintained automatically, and should purposefully be aimed at and worked for. The Kpelle people of Liberia of West Africa are known for their ad hoc local meetings called moots or community palavers, where the conflicting parties arrive at mediated settlements through the use of experienced traditional leader (Bob-Manuel 2006). Bob-Manuel (2006) further tells that among the Ndendeuli of Tanzania, grasroot actors play active roles in conflict solving by suggesting an agreement and get as far as pressurising the parties into accepting it. Pressurising can be done through talking or singing: shaming and ridiculing. This special method can be used in contexts where it is acceptable and in instances where the cause of the dispute is self- evident. 2.5 The importance of Grassroots actors Grassroots actors are well positioned to address matters of community building, and identity formation. This is so due to a number of reasons as noted by Wilson (2001). Firstly, grassroots actors are positioned within the communities that they are working. These places them in close proximity to each individual which builds trust, respect and confidence between those directly involved in the conflict. Wilson (2001) points out that the Volunteer conflict transformation project in the rural provinces of Rwanda was widely accepted because grassroots actors were integrated into the local planning processes which catered for developmental needs of the community. Traditional approaches focus on the psycho-social and spiritual dimension of violent conflicts and their transformation. This dimension tends to be underestimated by actors who are brought up and think in the context of western enlightenment (Dore 1995). Conflict transformation and peace-building is not only about negotiations, political solutions and material reconstruction, but also about reconciliation and mental and spiritual healing. Traditional approaches have a lot to offer in this regard. They do not only deal with material issues, reason and talk, but also with the spiritual world, feelings and non-verbal communication. Thus Dore (1995) further highlights that reconciliation as the basis for the restoration of communal harmony and relationships is at the heart of customary conflict resolution. When dealing with conflicts based in a Third World or non-Western society, action or interference from external factors, such as International Non-Government Organisations, is often regarded as culturally insensitive or an act of Western imperialism (Solomon and Mangqibisa 2000). This perception of outside interference affects the prospects of conflict transformation as external actors intervening in the conflict often have established methods of interaction that often disregard traditions of conflict resolution that are evident in conflict-ridden societies (Solomon and Mngqibisa 2000). Grassroots actors are however, often familiar with particularistic traditions of community-building. As these are more likely to be positively received by the people, traditional methods are more conducive to peace promotion and enduring stability and cooperation within the society. Traditional approaches are holistic, comprising also social, economic, cultural and religious-spiritual dimensions. This is in accordance with the entirety of traditional lifestyles and world views in which the different spheres of societal life are hardly separated. (Kubasu 2008)The conflict parties can directly engage in negotiations on conflict termination and in the search for a solution, or a third party can be invited to mediate; in any case the process is public, and the participation in the process and the approval of results is voluntary. It is carried out by social groups in the interest of social groups (extended families, clans, village communities, tribes, brotherhoods, etc.); individuals are perceived as members of a group, they are accountable to that group, and the group is accountable for (the deeds of) each of its members. Kubasu (2008) advances that grassroot action provides an invaluable contribution to the process and substance of conflict transformation. Through their position in the socio-political hierarchy, Traditional leaders are well placed to address issues of identity and may often initiate change in an environment generally un-conducive to larger scale attempts at conflict resolution. Whilst the activities engaged by grassroots actors is largely context specific, conflict transformation theorists and practitioners can look to the success of different grassroots initiatives to build upon the current literature. 2.5.1 Comparison between grassroots conflict transformation and Morden conflict resolution Table 1(Adopted from Kubasu 2008) Traditional conflict resolution values Morden Judgement handed down by the traditional leader whose throne is hereditary and his assessors selected on their merits Judgement handed down by judges whose office is conferred on them through formal qualifications Peaceful resolution and recourse to justice Frequent recourse to revenge and violence, without waiting for justice Concern to reconcile the parties in conflict Judgements are categorical. There is no concern to bring together the parties to the conflict, nor for any later outcome The traditional leaders act out of a spirit of honesty, impartiality and fairness They offer their services voluntarily Bureaucratic and (at times) corrupt mentality Paid work Justice by the people involving everyone (everyone can come to listen and give an opinion)at the dares or village circles Justice is the province of a particular group of people (those with formal qualifications) Lengthy judicial process Disputes settled quickly or over a period of time(transformative) Moral and social sanctions Physical punishment and material fines Torture and imprisonment(does not create social harmony) Divesting individuals of their functions Being discredited in others eyes Being marginalized Paying fines Once the penalty has been paid, no further reference made to offences committed 2.5.2 Conflict Mapping and Analysis Processes of conflict resolution in Africa are characterised by three dimensions which include the nature of conflicts, conflict resolution mechanisms and the outcome of such mechanisms. In understanding the nature of conflicts, first there is need to identify types of conflicts (Maruta and Mpofu: 2004). There have been different ways of identifying types of conflicts. One way is in terms of complexity. It has been observed that in Zimbabwe there are simple and complex types of conflicts (Alexander 1995). Most of the conflicts have been and continue to be complex. The second way is in terms of duration. In this context there are short lived and protracted conflicts. Protracted conflicts are the most common in rural Zimbabwe these include conflicts of resources especially land and well as ethnic or tribal issues .In the Midlands and Matabeleland were protracted conflicts and civil wars that came after independence . The third way is in terms of violence. There are conflicts which are violent and those which are non-violent. Some people have characterised the non-violent conflicts as latent or structured conflicts (Fisher 2007). However, most conflicts which have been studied and which have drawn greater attention are violent conflicts which have involved bloodshed. Although most conflict resolution measures have been taken on violent conflicts, there have also been situations when conflict resolution measures have been made on latent conflicts. For example the latent conflict between traditional leaders and elected councillors in the Zibabgwe district of Kwekwe gave rise to a process of peace negotiations under the auspices of the Centre for Conflict Management and Transformation (Mpangala, 2000). The fourth way of identifying types of conflicts is in terms of the scale of the conflict. In this context conflicts in rural areas have been categorised as either resource or political conflicts, with a few tribal and household conflicts 2.5.3 The effect of political involvement Sometimes the role of traditional leaders as champions of conflict transformation and good governance is compromised by their involvement in politics, this motivates the people to challenge their legitimacy and the validity of their judgements, and as such this affects their leadership capabilities. The Newsday(2012) reported that the institution of the traditional leadership has come under spotlight following the governments intention to bestow greater powers on the traditional leaders , the argument here is that the age old concept of traditional leaders remaining mere custodians of cultural values and interceding with the ancestors has been turned head on as a ruling government seeks to enhance their political expediency though influencing traditional leaders, Kubatana(2012) supports this by quoting President Mugabes speech when he said chiefs should no longer remain repositories of oral history You should be guardians of our national sovereignty and guard against those who delight in associating with our detractors and those who work in cahoots with the powers that seek to mislead our people(Kubatana :2012) Mararike (2011) observes that although there is need to improve the role and operations of chiefs , greater caution should be taken to prevent abuse of any authority guaranteed , traditional leaders are unable to operate effectively because of the dualism of using the Roman Dutch Law as the basis of our legal system and the traditional system hence there could be serious problems unless the traditional system of government is clearly separated from the political party system whereby traditional leaders are separated in a non-partisan way Few traditional leaders have legal remaining to despise Morden forms of justice their judgement and authority can be easily contested and overlooked, the Chief Negomo vs. the Prime minister Tsvangirai issue is one such example of a situation where traditional leaders are oblivion to address community issues but go on to fight the politicians, however they end up with their decisions overlooked and their legitimacy questioned, Mararike (2011) further argues that the current crop of traditional leaders have no capacity , he argues that young ,educated and professional men should be appointed as chiefs otherwise the current crop would need support staff to dispense justice without biases among rural communities. 2.6 The Traditional Conflict Transformation approaches During the years of traditional leadership in Africa various conflicts caused by different issues attracted various approaches to their resolution. Most conflicts and their resolution methods at that time were predominantly local. Conflicts were between individuals, villages, communities or tribes who lived in the same or adjoining areas. Those who intervened were often local elders and /or tribal leaders. When kingdoms developed about the 17th and 18th century in southern Africa, stronger and wider authority came into power, but the traditional methods of instigating and resolving conflicts had gone through very small changes and are now gradually starting their process of decline. 2.6.1 Mediation Mediation is defined as an attempt to settle a dispute through an active participation of a third party(Mediator) who works to find points of agreement and make those in conflict agree on a fair result. The Harvard journal of Conflict Studies (Nov 2008) defines mediation as an act of reconciliation that is trying to unite and reach an agreement between conflicting parties. The chiefs and headman are respected as trustworthy mediators all over Africa, because of their accumulated experience and wisdom as they are usually of an elderly age. Their role as mediators would depend on traditions, circumstances and personalities, accordingly of their society. These roles include: pressurising or manipulating conflicting parties to reach an agreement, making recommendations, giving assessment, conveying suggestions on behalf of a party. Behaviour used is facilitation, through clarifying information, promoting clear communication, interpreting standpoints, summarising discussions, emphasising relevant norms or rules, envisaging the situation if agreement is not reached, or repeating of the agreement already attained. The mediators can also remain passive, as they are there to represent important shared values. There is no predetermined model, so they are entitled to change their roles fr